八波研究室

論文

• R. Yatsunami, A. Ando, N.Miyoko, Y Yang, S. Takaichi and S.Nakamura. "Two distinct enzymes have both phytoene desaturase and 3,4-desaturase acitivites involved in carotenoid biosynthesis of extremely halophilic archaeon Haloarcula japonica", M&E, in press

　

• Y. Hagiwara, T. Okeda, K. Okuda, R. Yatsunami, S. Nakamura. “Characterization of a xylanase belonging to the glycoside hydrolase family 5 subfamily 35 from Paenibacillus sp.H2C” Biosci, Biotechnol, Biochem., 87, 54-62, 2023. doi: 10.1093/bbb/zbac175




• S. Nagaoka, N. Sugiyama, R. Yatsunami, S. Nakamura. “Characterization of 3-isopropylmalate dehydrogenase from extremely halophilic archaeon Haloarcula japonica” Biosci, Biotechnol, Biochem., 85, 1986-1994, 2021. doi: 10.1093/bbb/zbab122




• K. Kuwata, M. Suzuki, T. Takita, R. Yatsunami, S. Nakamura, and K. Yasukawa. “The mutation of Thr315 to Asn of GH10 xylanase XynR increases the alkaliphily but decreases the alkaline resistance” Biosci, Biotechnol, Biochem., 85, 1853–1860, 2021. doi:10.1093/bbb/zbab102




• Y. Makino, H. Sato, A. Noguchi, T. Ide, R. Yatsunami, and S. Nakamura. “Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli” Biosci, Biotechnol, Biochem., 85, 1464-1467, 2021. doi: 10.1093/bbb/zbab057




• R. Sueda, K. Yoshida, M. Onodera, T. Fukui, R. Yatsunami and S. Nakamura, “Characterization of a GlgC homolog from extremely halophilic archaeon Haloarcula japonica” Biosci, Biotechnol, Biochem. 85, 1441–1447,2021. doi:10.1093/bbb/zbab050




• A. Kaneda, T. Seike, T. Danjo, T. Nakajima, N. Otsubo, D. Yamaguchi, Y. Tsuji, K. Hamaguchi, M. Yasunaga, Y. Nishiya, M. Suzuki, J. Saito, R. Yatsunami, S. Nakamura, Y. Sekido, K. Mori. “The novel potent TEAD inhibitor, K-975, inhibits YAP1/TAZ-TEAD protein-protein interactions and exerts an anti-tumor effect on malignant pleural mesothelioma” Am. J. Cancer Res., 10, 4399-4415, 2020.




• K. Okuda, K. Ito, Y. Hagiwara, T. Okeda, R. Yatsunami, T. Fukui and S. Nakamura. “Characterization of novel arabinofuranosidases from Paenibacillus sp. strain H2C” J. Jpn. Soc.Extremophiles 16, 37-45, 2020.




• Y. Hagiwara, Y. Mihara, K. Sakagami, R. Sagara, U. Bad-Erdene, R Yatsunami, S. Nakamura. “Isolation of four xylanases capable of hydrolyzing corn fiber xylan from Paenibacillus sp. H2C” Biosci, Biotechnol, Biochem. 20, 1-11, 2019. doi: 10.1080/09168451.2019.1693253.




• T. Takitaa, K. Nakatania, Y. Katanoa, M. Suzukia, K. Kojimaa, N. Sakab, B. Mikamib, R. Yatsunami, S. Nakamurac, and K. Yasukawaa “Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis” Enzyme Microb. Tech., 130,109363, 2019. doi: 10.1016/j.enzmictec.2019.109363.




• K. Nakatani, Y. Katano, K. Kojima, T. Takita, R. Yatsunami, S. Nakamura and K. Yasukawa. “Increase in the thermostability of Bacillus sp. strain TAR-1 xylanase using a site saturation mutagenesis library” Biosci. Biotechnol. Biochem., 82, 1715-1723, 2018. doi: 10.1080/09168451.2018.1495550.




• K. Toyama, D. Misu, R. Kajitani, K. Endo, T. Fukazawa, R. Yatsunami, T. Ito, T. Fukui and S. Nakamura “Characterization of a novel multi-domain chitinase from alkaliphilic Nocardiopsis sp. strain F96” J. Jpn. Soc. Extremophiles, 16, 37-45, 2017.




• K. Endo, K. Nagao, K. Toyama, S. Ishida, T. Fukazawa, R. Yatsunami, T. Fukui, X. Xing and S. Nakamura. “Cloning of a chitinase gene from newly isolated alkaliphilic Nocardiopsis sp. strain F96 and extracellular production of the recombinant protein in Escherichia coli” J. Jpn. Soc. Extremophiles, 14, 21-29, 2015.




• Y. Yang, R. Yatsunami, A. Ando, N. Miyoko, T. Fukui, S. Takaichi, and S. Nakamura. “Complete Biosynthetic Pathway of the C50 Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica”J. Bacteriol., 197, 1614-1623, 2015.




• R. Yatsunami, A. Ando, Y. Yang, S. Takaichi, M. Kohno, Y. Matsumura, H. Ikeda, T. Fukui, K. Nakasone, N. Fujita, M. Sekine, T. Takashina, and S. Nakamura, “Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica” Front. Microbiol., 5, 100-105, 2014,. doi: 10.3389/fmicb.2014.00100.




• T. Tadikara, T. Matsubara, Y. Kubota, T. Kosaka, T. Ozawa, W. Tsukimura, R. Yatsunami, T. Fukui, K. Nakasone, T. Takashina and S. Nakamura “Gene expression and characterization of aerotaxis transducer HemAT from extremely halohilic archaeon Haloarcula japonica TR-1” J. Jpn. Soc. Extremophiles.12, 29-32, 2013




• M. Onodera, R. Yatsunami, W. Tsukimura, T. Fukui, K. Nakasone, T. Takashina and S. Nakamura “Gene analysis, expression and characterization of an intracellular α-amylase from extremely halophilic archaeon Haloarcula japonica” Biosci. Biotechnol. Biochem., 77, 281-288, 2013.




• S. Chen, Z. Huang, J. Wu, Y. Chen, F. Ye, C. Zhang, R. Yatsunami, S. Nakamura and X.-H. Xing “Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli.” Appl. Microbiol. Biotechnol. , 97, 2907-2919, 2013, doi: 10.1007/s00253-012-4145-6.




• F. Uni, S. Lee, R. Yatsuanmi, T. Fukui and S. Nakamura “Mutational Analysis of a CBM Family 5 Chitin-Binding Domain of an Alkaline Chitinase from Bacillus sp. J813” Biosci. Biotechnol. Biochem., 76, 530-535, 2012.




• 八波　利恵 “高度好塩性古細菌に由来する機能性タンパク質の解析” J. Jpn. Soc. Extremophiles, 10, 17-22, 2011.




• S. Chen, F. Ye, Y. Chen, Y. Chen, H. Zhao, R. Yatsunami, S. Nakamura, F. Arisaka and X.-H. Xing “Biochemical Analysis and Kinetic Modeling of the Thermal Inactivation of MBP-Fused Heparinase I: Implications for a Comprehensive Thermostabilization Strategy” Biotechnol. Bioeng., 108, 1841-1851, 2011.




• R. Yazawa, J. Takakura, T. Sakata, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura. “A Calcium-Dependent Xylan- Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1” Biosci. Biotechnol. Biochem., 75, 379-381, 2011.




• Y. Zhang, R. An, R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, T. Fukui, and S. Nakamura. “Characterization of a Haloarchaeal GH family 18 Chitinase with Additional Acidic Amino Acids on Its Protein Surface” J. Jpn. Soc. Extremophiles, 9, 72-74, 2010.




• R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, Y. Zhang, T. Takashina, T. Fukui, and S. Nakamura. “Gene expression and characterization of a novel GH family 18 chitinase from extremely halophilic archaeon Halobacterium salinarum NRC-1” J. Jpn. Soc. Extremophiles, 9, 19-24, 2010.




• W. Tsukimura, K. Watanabe, C. Morokuma, R. Yatsunami, T. Fukui, and S. Nakamura. “Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution” J. Jpn. Soc. Extremophiles, 9, 15-18, 2010.




• H. Umemoto, R. Yazawa, J. Takakura, R. Yatsunami, T. Fukui and S. Nakamura. “Analysis of functional domains and improvement of alkaliphily of an alkaline xylanase on the basis of its three-dimensional structure” J. Appl. Glycosci., 57, 145-150, 2010.




• H. Umemoto, Ihsanawati, M. Inami, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura. “Improvement of Alkaliphily of Bacillus Alkaline Xylanase by Intrducing Amino Acid Substitutions Both on Catalytic Cleft and Protein Surface”. Biosci. Biotechnol. Biochem., 73, 965-967, 2009.




• N. Koizumi, S. Matsuda, K. Maeda, Y. Isoda, R. Yatsunami, T. Fukui and S. Nakamura. “Additional Carbohydrate-Binding Module Enhance the Insoluble Substrate-Hydrolytic Activity of β-1,3-glucanase from Alkaliphilic Nocardiopsis sp. F96.” Biosci. Biotechnol. Biochem., 73, 1078-1082, 2009.




• S. Masuda, K. Endo, N. Koizumi, T. Hayami, T. Fukazawa, R. Yatsunami, T. Fukui and S. Nakamura. “Molecular Identification of a Novel Beta-1,3-glucanase from Alkaliphilic Nocardiopsis sp. Strain F96.” Extremophiles, 10, 251-255, 2006.




• T. Sakata, H. Miyakubo, Y. Osada, R. Wada, H. Takahashi, R. Yatsunami, T. Fukui and S. Nakamura. “Functional Improvement of Xylanase by Introducing Mutated Xylan-binding Domain.” J. Appl. Glycosci., 53, 131-136, 2006.




• Ihsanawati, T. Kumasaka, T. Kaneko, C. Morokuma, R. Yatsunami, T. Sato, S. Nakamura and N. Tanaka. “Structural Basis of the Substrate Subsite and the Highly Thermal Stability of Xylanase 10B from Thermotoga maritima MSB8.” Proteins, 61, 999-1009, 2005.




• K. Ozawa, T. Harashina, R. Yatsunami and S. Nakamura. “Gene cloning, expression and partial characterization of cell division protein FtsZ1 from extremely halophilic archaeon Haloarcula japonica strain TR-1.” Extremophiles, 9, 281-288, 2005.




• N. Hirota, T. Matsuo, A. Ikeda, R. Yatsunami, T. Fukui and S. Nakamura. “Role of an N-terminal domain found in the ferredoxin from extremely halophilic archaeon Haloarcula japonica.” J. Jpn. Soc. Extremophiles, 4, 14-24, 2005.




• A. Takenaka, W. Adachi, Y. Sakihama, S. Shimizu, T. Sunami, T. Fukazawa, M. Suzuki, R. Yatsunami, and S. Nakamura. “Various Chitosanase Developed from Different Structural Kingdoms. Chitin Chitosan Res.,” 11, 1-13, 2005.




• W. Adachi, S. Shimizu, T. Sunami, T. Fukazawa, Y. Sakihama, M. Suzuki, R. Yatsunami, S. Nakamura and A. Takenaka. “Crystal Structure of Family GH-8 Chitosanase with Subclass II Specificity from Bacillus sp. K17.” J. Mol. Biol., 343, 785-795, 2004.




• Y. Sakihama, W. Adachi, S. Shimizu, T. Sunami, T. Fukazawa, M. Suzuki, R. Yatsunami, S. Nakamura and A. Takenaka. “Crystallizations and Preliminary X-ray Analyses of the Active and the Inactive Forms of Family GH-8 Chitosanase with Subclass II Specificity from Bacillus sp. Strain K17.” Acta Cryst., D60, 2081-2083, 2004.




• K. Endo, T. Fukazawa, R. Yatsunami and S. Nakamura. “Molecular Cloning of a Family 18 chitinase gene from alkaliphilic Nocardiopsis sp. strain F96. Chitin Chitosan Res, 9, 150-151, 2003.




• T. Matsuo, A. Ikeda, N. Hirota, D. Sugimori, R. Yatsunami and S. Nakamura. “Cloning and Expression of Haloarcula japonica Ferredoxin Gene, and Characterization of Recombinant Proteins.” Porphyrins. 11, 108-108, 2002.




• R. Yatsunami, Y. Sakihama, M. Suzuki, T. Fukazawa, S. Shimizu, T. Sunami, K. Endo, A. Takenaka and S. Nakamura “A Novel Chitosanase from Bacillus sp. Strain K17: Gene Cloning and Expression in Escherichia coli” Nucleic Acids Res. Suppl, 2, 227-228, 2002.




• R. Yatsunami, M. Iwamoto, K. Ihara and S. Nakamura “Gene Clusters Encoding ATP synthase of Haloarcula japonica Strain TR-1” Nucleic Acids Res. Suppl., 1, 51-52, 2001.




• R. Yatsunami, T. Kawakami, H. Ohtani and S. Nakamura. “A novel bacteriorhodopsin-like protein from Haloarcula japonica strain TR-1: Gene cloning, squencing and transcript analysis. Extremophiles, 4, 109-144, 2000.




• R. Yatsunami, S. Aono and S. Nakamura “The Gene Encoding a Novel Halorhodopsin-like Protein of Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1” Nucleic Acids Symp. Ser. , 44, 1-2, 2000.




• K. Ozawa, R. Yatsunami and S. Nakamura “Cloning and Sequencing of ftsZ Homolog from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1” Nucleic Acids Symp. Ser. , 44, 155-156, 2000.




• R. Yatsunami, T. Kawakami, H. Ohtani and S. Nakamura “A Novel Bacteriorhodopsin-like Protein from Haloarcula japonica Strain TR-1: Gene Cloning, Sequencing and Transcript Analysis” Extremophiles, 4, 109-144, 2000.




• R. Yatsunami, T. Kawakami, H. Ohtani and S. Nakamura “Transcriptional Regulation of Cruxrhodopsin Gene from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1” Nucleic Acids Symp. Ser. , 42, 73-74, 1999.




• R. Yatsunami, M. Iwamoto, K. Ihara and S. Nakamura “Molecular Cloning of A1-ATPase Genes from Extremely Halophilic Archaeon Haloarcula japonica Strain TR-1” Nucleic Acids Symp. Ser. , 42, 75-76, 1999.




• R. Yatsunami, T. Kawakami, H. Ohtani and S. Nakamura “Primary Structure of the Novel Bacterial Rhodopsin from Extremely Halophilic Archaea Haloarcula japonica Strain TR-1” Nucleic Acids Symp. Ser. , 37, 11-112, 1997.

学術雑誌等又は商業誌における解説、総説

• 八波利恵， "高度好塩性古細菌Haloarcula japonicaのイソプレノイド生合成経路の解析と代謝工学による有用物質生産"日本応用酵素協会誌,57.27-32,2022.




• 八波利恵， “古細菌のカロテノイド生合成経路” 生物工学, 93, 394-396, 2015.




• 八波利恵， “極限環境微生物が合成するカロテノイド：バクテリオルベリン” 生物工学会誌, 90, 738, 2012.




• 八波利恵， “高度好塩性古細菌に由来する機能性タンパク質の解析” 極限環境生物学会誌, 10, 17-22, 2011.




• Y. Zhang, R. Yatsunami, Y. Hatori, M. Sato, K. Orishimo, T. Fukui and S. Nakamura “Halotolerancy and Organic-Solvent Tolerancy of Haloarchaeal Chitinase” Biotechnology of Lignocellulose Degradation, Biomass Utilization and Biorefinery (Ed. By K. Sakka et al), Ito Print Publishing Division, 442-447, 2008.




• W. Tsukimura, K. Watanabe, C. Morokuma, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura “Improvement of Alkaliphily of Family 10 Xylanase from Hyperthermophilie Thermotoga maritime MSB8” Biotechnology of Lignocellulose Degradation, Biomass Utilization and Biorefinery (Ed. By K. Sakka et al), Ito Print Publishing Division, 423-427, 2008.




• R. Yazawa, J. Takakura, T. Sakata, H. Miyakubo, Ihsanawati, H. Umemoto, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura “Mutational Analysis of CBM Family 36 Xylan-binding Domain of Alkaline Xylanse” Biotechnology of Lignocellulose Degradation, Biomass Utilization and Biorefinery (Ed. By K. Sakka et al), Ito Print Publishing Division, 448-454, 2008.




• W. Adachi, S. Shimizu, T. Sunami, T. Fukazawa, M. Suzuki, R. Yatsunami, S. Nakamura and A. Takenaka “Evolutionary Classification of Family 8 Glucoside Hydrolases, Based on the X-ray Structure of Bacillus sp. K17 Chitosanase” Genetics, Biochemistry and Ecology of Cellulose Degradation (ed. by K. Ohmiya, et al), Uni Publishers Co., Ltd., 52-55, 2004.




• Y. Sakihama, M. Suzuki, T. Fukazawa, R. Yatsunami, K. Endo, W. Adachi, S. Shimizu, T. Sunami, A. Takenaka and S. Nakamura “A Novel Chitosanase from Bacillus sp. Strain K17: Purification, Characterization and Gene Cloning” Genetics, Biochemistry and Ecology of Cellulose Degradation (ed. by K. Ohmiya, et al), Uni Publishers Co., Ltd., 478-483, 2004.




• S. Masuda, K. Endo, T. Hayami, T. Fukazawa, R. Yatsunami and S. Nakamura “Characterization of beta-1,3-Glucanases from Alkaliphilic Nocardiopsis sp. Strain F96” Genetics, Biochemistry and Ecology of Cellulose Degradation (ed. by K. Ohmiya, et al), Uni Publishers Co., Ltd., 495-500, 2004.




• 八波利恵，中村　聡 “好塩菌に備わった分子ポンプ” 化学装置, 4, 66-68, 2003.




• 八波利恵，中村　聡 “生体分子と自己修復” 化学と教育, 49, 122-123, 2000.




• 八波利恵，中村　聡 “SINE 配列に学ぶ生命の進化” 化学と生物, 8, 523-524, 1998.




• 八波利恵，中村　聡 “クジラ DNA に刻まれた生命の起源” 化学と工業, 6, 918, 1998.

著書

• （分担執筆）八波利恵,　“第3節　高度好塩性アーキアのカロテノイド生合成経路”,　第2章　極限酵素/タンパク質の機能解析,　極限環境微生物の先端科学技術と社会実装前線　(伊藤政博,　鳴海一成,　道久則之・監修),　エヌ・ティー・エス,　2023.




• （共著）中村聡，中島春紫，伊藤政博，道久則之，八波利恵， “第7章微生物の増殖，第9章免疫学的手法，第12章各種汎用機器の取り扱い”，新版ビギナーのための微生物実験ラボガイド，講談社，2019.




• （分担執筆）八波利恵，“高度好塩性アーキアのエネルギー転換系代謝”，第6章アーキアにおける物質変換, アーキアの生物学（日本Archaea研究会・監修）共立出版，151-154, 2017.




• （分担執筆）八波利恵，中村　聡. “第5編　酵素を操る, 第4章　酵素の機能改変-方法論，第1節　部位特異的変異導入法による酵素の機能改変” 酵素利用技術大系（小宮山眞・監修）, エヌ・ティー・エス, 2010.




• （分担執筆）八波利恵，中村　聡. “酵素パワーで汚れを落とす：洗剤添加用酵素” トコトンやさしいタンパク質の本（東京工業大学大学院生命理工学研究科・編），日刊工業新聞社，2007.